Using the SoftMax Pro Workflow Editor, a kinetic cycle containing an absorbance read (PlateOD600) and a fluorescence read (PlateGFPBottom) with a 5-second linear plate shake between reads was created (Figure 1). Table 1. SpectraMax Microplate Readers. Align the two slots on the bottle holder with the two pegs on the rail. Multimode Reader Comparison Chart. The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with added functaionlity of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. 0 mm hex key to remove the six blue retaining screws that secure the transport bracket (B) to the instrument. 0´ 104 8. User-upgradeable detection modules allow you to expand the capabilities of your microp. The SpectraMax i3x reader provides the The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. Expand your lab’s capabilities to include flash applications such as dual luciferase and. Dilution series of HeLa cells transfected with firefly (red plot) and Renilla (blue plot) luciferases. The StemoniX microBrain 3D platform is a homogenous 3D neural spheroid system in 96 and 384-well format. The SpectraMax i2X is used for cell migration, cell. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Real-time measurement of extracellular oxygen depletion and acidification in cultured cells and microorganisms. Explore more about Optimized settings for Transcreener TR-FRET assay on SpectraMax iD5 and SpectraMax i3x readers. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. The SpectraMax® DuoLuc™ Reporter Assay Kit enables highly sensitive quantitation of both firefly and Renilla luciferases in mammalian cells at a great price. BRET2canbeusedtostudyvariouskindsofprotein-proteininteractions. Methods. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. Explore PDF for more details. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellThis characterization of nanoparticle and its surface interactions can be detected using the SpectraMax i3x Multi-Mode Microplate Reader and the Spectral Optimization Wizard in SoftMax Pro Software. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast. Products : SoftMax Pro GxP Software, SoftMax Pro Software. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. detection cartridge LUM96: 59. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. . The SpectraMax M4 reader had identical performance specifications to the SpectraMaxLuminescence was read on the SpectraMax i3x reader and the SpectraMax L reader. iD5. In the second assay, HepG2 cells were plated into a 96-well clear-bottom, blackwalled plate at 10,000 cells/well and incubated overnight at 37°C. GloMax ® Navigator System. SpectraMax i3x Multi-Mode Microplate Reader; SpectraMax MiniMax 300 Imaging Cytometer *Here, the NucView 488 with DMSO formulation was used. Spectramax I3x, supplied by Danaher Inc, used in various techniques. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. . Dual auto injector cartridges for the SpectraMax® i3x Multi-Mode Microplate Detection Platform allows you to expand your research capabilities to include flash based applications, including dual luciferase and ATP assays. Transfected cells were imaged on the SpectraMax MiniMax 300 Imaging Cytometer. Background noise is reduced by the reader’s cooled PMT, enabling increased sensitivity and dynamic range. The SpectraMax i3x Multi-Mode Detection Platform User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax i3x, a versatile and customizable microplate reader that can perform various assays and imaging tasks. 6% for the on-board optics (monochromators). Structured Review. 3 Figures from 612 Publications Promega. Wokingham, Berkshire RG41 5TS United Kingdom. Prepare your i3/i3x instrument for shipment. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. SpectraMax i3x readers. Tip: MCF-7 cells have a polygonal morphology with irregular dimensions, and they have a habit of clumping into large aggregates. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. The heating element design of the category 1 microplate readers, such as the SpectraMax i3x Multi-Mode Microplate Reader, applies a dual area sensor for temperature control. As. The possibility of user upgrading the system with an application cartridge and an imaging cytometer makes this device the most flexible microplate. . no. Find regional support. For each assay kit, a preconfigured protocol is included in SoftMax Pro Software to facilitate the acquisition and analysis of data for a simplified workflow. After 2 days of incubation, LUC and REN activities were measured using a SpectraMax ® i3x Multi-Mode detection platform (Molecular Devices, USA) with a Dual-Luciferase Reporter Assay Kit (Promega. 5 µL anti-cAMP-Cryptate. ValitaTiter (i3x i3) ValitaTiter (iD5) ValitaTiter (M5 M5e Flex3) ValitaTiter (Paradigm) ValitaTiter (FilterMax F5) Assay-Specific. Core features include biological and fume hoods, a cold room and multiple freezers, and access to institutional space for radiolabeling and radiation monitoring. using the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. Supplier: Molecular Devices. 1 and above: Compatible with only with 64-bit OS. Download PDF. Fluorescence measurements of the soil solutions and enzyme activities were made using a microplate reader (SpectraMax i3x, Molecular Devices, USA). Molecular Devices, LLC Reading Modes: Endpoint and Kinetic; Optical System: Spectral Fusion Illumination. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Prepare your iD series instrument for shipment. 306 plate air cal. Note: Additional readers with similar performance are listed. Keywords: filtermax multi mode microplate readers, spectramax paradigm multimode detection platform, spectramax paradigm system Created Date: 11/16/2010 12:21:51 PMThe SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. each new lot of plates, to ensure that the center of each well is read. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. The SpectraMax Paradigm Multi-Mode Detection Platform is a modular multi-mode microplate detector. application modules • Multi-mode microplate reader and imaging system • Wide dynamic range • StainFree™ cell counting technology • Library of user-configurable applications • Advanced curve fitting and statistical analysis • Cell confluence and cell viability Introduction. Nucleic Acid and Protein Quantitation. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. . Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). In brief, a pre-titrated dose of virus was incubated with 8 serial 5-fold dilutions of serum samples in duplicate in. SpectraMax i3x; SpectraMax iD3 / iD5; SpectraMax M Series; SoftMax Pro Software; CELLULAR IMAGING. ZERO BIAS - scores, article reviews, protocol conditions and more. ValitaTiter Plus settingsContact our support team with your questions regarding Molecular Devices products and applications. Spectranet customer portal. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideRecommendations for SpectraMax i3/i3x plate readers The Molecular Devices SpectraMax i3/i3x allows use of the pH-Xtra Glycolysis Assay with time-resolved fluorescence (TRF) detection. Multimode Reader Comparison Chart. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. applications, including dual luciferase and ATP assays. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. The calculated LOQ values were 0. Instrument. Multiple automated tests are provided including: • Linearity • Precision • Accuracy • Dynamic range • Alignment • Signal to noise • Signal to background • Filter checks. Monday to Friday 8am - 5pm GMT. 99 to $1999. Replicate values were averaged and imported into Design-Expert 8. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection. Spectramax I3x, supplied by Danaher Inc, used in various techniques. SpectraMax M3 Plate Read, Plate Map, Data Analysis and Export Setup pg. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Molecular Devices. Using the SpectraMax i3x reader, the preconfigured SpectraMax Glo Steady-Luc Reporter assay protocol in SoftMax Pro Software was used to measure luminescence. Background Parallax,Columns 2. g. Flash-type luminescence with excellent sensitivity Expression measurement in as few as ten cells per well Improved accuracy of results by. QuickSync. We would like to show you a description here but the site won’t allow us. Molecular Devicesの全商品を見る. . eu@moldev. Viewing this answer requires an authorized account. Figure 1. Overview of Western Blot Cartridge. SpectraMax ® iD3 and iD5 Multi-Mode . For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. Danaher Inc spectramax i3x. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax ® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Unparalleled performance on a personalized platform. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email drugdiscoverytech@lifetech. 98 / 100 stars. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. Spectramax I3x, supplied by Danaher Inc, used in various techniques. See SpectraMax® Microplate Reader User Manual Download Page. It allows you to explore cellular pathways and protein activation and expression in one system. . This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. MC38 in vivo tumor model in hPD-1. 99. Whether you are looking for an intuitive, easy-to-use reader for. モジュールを追加してアップグレードすることにより、ウェスタンブロットやイメージング、試薬インジェクタを用いた高速カイネティック測定を行う. i3x single use: $35: $52. Reaction catalyzed by the firefly luciferase. Filterless readers provide consistent results with steady temperature regulation. 2 Parallel Line Analysis Conc log [ng/ML] RLU. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. i3x Specifications. The chamber. The calculated LOQ values were 0. which chemiluminescence was detected on a SpectraMax i3x reader (Molecular Devices) and analyzed us ing GraphPad. 2 Parallel Line Analysis Conc log [ng/ML] RLU. Table 1. FlexStation® 3 Multi-Mode Microplate Reader. Microplate optimization should be performed the first time the assay is run, and subsequently for . Using optimized settings for the Transcreener FP assays presented in this application note, the SpectraMax i3x. Facilities. 58% for the detection module and 2. The NET-specificity of the detected signal was determined by incubation with DNase I (50 U/mL, Qiagen, Germany). 더 많은. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Figure 3. Read modes. 030 fmol. )wideand65. The SpectraMax i3x reader provides theSoftMaxProSoftwareFormulaReferenceGuide 8 5049093A SummaryFormulas Summariesconsistoffourparts: ASummaryname(whichisusedtorefertotheSummaryinotherformulas). Using the SoftMax Pro Workflow Editor, a kinetic cycle containing an absorbance read (PlateOD600) and a fluorescence read (PlateGFPBottom) with a 5-second linear plate shake between reads was created (Figure 1). Ease of use. Negative samples contained only MycoAlert Assay Buffer. ClonePix 2 Mammalian Colony Picker; QPix Microbial Colony Pickers; CloneSelect Imager; DispenCell Single-cell Dispenser; 3D. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. 1. Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Cet appareil ISM est confomre à la norme NMB-001 du Canada. 0´ 105 dsD NA (p g/ wel ) R F UFluorescence intensity was measured using a Spectramax i3x plate reader (Molecular Devices) with excitation at 575 nm (bandwidth 9 nm) and emission at 608 nm (bandwidth 15 nm). User-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. 302 low ref light. Table 1. HTRF IP standard curve. For wavelengths in the UV range above 220 nm,. Winnersh Triangle. Engineered for high performance, the auto dual injector cartridge includes fail safe features. It can be upgraded with. Italic and boldface type are used for emphasis. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologyMeasure oxidative metabolism and glycolytic activity on the SpectraMax i3x reader. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Controls containing all reaction. This technique allows for quality control and assurance when performing surface functionalization of nanoparticles. . 2 nM was calculated using SoftMax Pro Software. This update is applicable for users of the SpectraMax® i3x Multi-Mode Detection Platform and SpectraMax® Paradigm® Multi-Mode Microplate Reader with the ScanLater Western Blot (WB) Detection Cartridge and the SpectraMax® iD5 Multi-Mode Microplate Reader: SMP 7. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. . With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Contact Local Team. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. were performed on a Biomek i7 containing an integrated orbital plate shaker and a SpectraMax i3x plate reader (Molecular Devices). The chamber. (SpectraMax i3x and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most published microplate reader control and microplate data analysis software package available to scientists. 001 0. We would like to show you a description here but the site won’t allow us. 吸収度マイクロプレートリーダー SpectraMax® Mini. 58% for the detection module and 2. The SpectraMax® i3x offers a wealth of applications; it can measure fluorescence, absorbance and luminescence, and its modular upgrades enable western blotting, imaging cytometry and injectors. The Immunochemical Core includes approximately 2,600 square feet of space in the Joseph Building at Mayo Clinic Hospital, Saint Marys Campus. Examples: “Press carefully to engage,” “ Do not press down. Bioz Stars score: 98/100, based on 1 PubMed citations. The information is below the horizontal line. Find regional support. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence polarization, and time-resolved. EUROPE. Check software compatibility with Windows 10 and locate your software installer. User Manuals, Guides and Specifications for your Molecular Devices SpectraMax i3x Measuring Instruments. Itemploysa. Automated for ease of use. SpectraMax® Microplate Readers and SoftMax® Pro Software. to View Figures. Transcreener® HTS is a universal, high throughput biochemical assay platform based on the detection of nucleotides, which are formed by thousands of cellular enzymes—many of which catalyze the covalent regulatory. SpectraMax i3x offers all the techniques you need to explore cell pathway in a single system. compound concentration, and an IC 50 value of 21. Molecular Devices are known for both the reliability and the wide uses of their products. 과 같은 Parameter를 검사하여 평가할 수 있습니다. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. to View Figures. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Future ready The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular. 0´ 104 1. The 4x washing steps were also performed on deck using the Span-8 aspirate and dispense for the first three washes. Pancreatic lipase activity was determined by measuring the absorbance of the test solution at 405 nm with a micro plate reader (MD SpectraMax i3x). Published Dec 16, 2020 | Updated Oct 10, 2023. Assay setup for a 384-well low-volume plate. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular. SpectraMax i3x Multi-Mode Microplate Reader. D 1 1. Upon completion of a plate read, data are delivered to your computer in an Excel-compatible format. 1. 7 Figures from 1126 Publications. • SpectraMax i3x • Onboard luminescence • Detection cartridge (LUM 96) • SpectraMax iD5 • SpectraMax L • SpectraMax M5e • SpectraMax Mini. ü; Instrument Diagnostics Mode (for SpectraMax® i3x, SpectraMax® iD3, SpectraMax® iD5, SpectraMax® Paradigm)Absorbance was measured on the SpectraMax i3x Multi-Mode Platform at 475 nm and 660 nm after 1-4 hours of incubation. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 72 5039228D. Open the catalog to page 2. Faster data acquisition and analysis with preconfigured SoftMax Pro protocols. +1 800-635-5577. The SpectraMax i3x reader features a cooled photomultiplier tube (PMT) for low background noise in luminescence. Restart the system. The corrected absorbance is within 5% of the. com. Unlimited breadth of. 0. This eBook shows how to see more and do more with an imaging cytometer that enables rapid imaging and cell analysis. When used in combination with the SpectraMax Injector Cartridge, this system enables a wealth of flash type luminescence applications including reporter gene and other assays with exceptional sensitivity and dynamic range. Supplier: Molecular Devices. BMG LABTECH VANTAstar. Download PDF. 6% for the on-board optics (monochromators). ATP (“flash” luminescence using . Each heating element is independently. We would like to show you a description here but the site won’t allow us. . the SpectraMax iD3 is a fantastic plate reader for a wide range of assays. The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with. 1 Microplate Data Acquisition & Analysis Software for Windows. Using the SpectraMax i3x reader, the preconfigured SpectraMax Glo Steady-Luc Reporter assay protocol in SoftMax Pro Software was used to measure luminescence. SpectraMax i3x plate reader (Molecular Device, contact supplier directly) Vacuum pump system (e. 99 to $1999. The SpectraMax® iD3 Multi-Mode Microplate Reader and the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices® have a touch screen interface that provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. In order to calculate a humification index (HIX), spectra were obtained with an excitation wavelength of 254 nm over the emissions waveband of 300–480 nm at increments of 5 nm (Ohno 2002. Clamp for injector 1 is on the left and clamp for injector 2 is on the right. Read more about HTRF SpectraMax Detection Cartridges for SpectraMax i3x & Paradigm Systems which utilize a high energy Xenon flash lamp for the sensitive reading of HTRF reagents. SpectraMax i3x and Paradigm readers require the FP-FLUO. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. style. SpectraMax MiniMax 300 细胞成像系统 为 SpectraMax i3x 多功能微孔板检测平台而打造！ • • • • 主要特点 系统全面兼容细胞成像 及微孔板检测技术 独特无标记分析技术无需 对细胞染色即可进行细胞 计数和贴壁度的计算 具有多种检测功能可对 细胞活力及毒性进行全. They include the SpectraMax® iD3 Multi-Mode Microplate Reader, SpectraMax® L Microplate Reader, SpectraMax® i3x Multi-Mode Microplate Reader, and SpectraMax® M5 Multi-Mode Microplate Reader. 6% for the on-board optics (monochromators). . These robust readers have been placed in labs from Antarctica to the. 2in. Promega. Qualify the absorbance performance of SpectraMax® iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, and FlexStation 3 readers. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). The user guide also covers the features and specifications of the SpectraMax MiniMax 300. SpectraMax i3/i3x readers must be equipped with a suitableThe SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change. A: percent GFP-positive cells calculated using the classification feature in the software. 003 fmol 0. R8362. 0001 0. If no sound is heard during initialization, contact technical support. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Monochromator optics with adjustable bandwidth offer excellent sensitivity down to 6 nM tryptophan, as well as precise determination of the fluorescence emission peak shift that occurs upon protein denaturation. Description. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for. SpectraMax M5. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. The dynamic range of the assay is about 3. NORTH AMERICA. Microplate and read height should be optimized for each new lot of microplates or assay volume used (use the Show Pre-Read Optimization options in the instrument settings in SoftMax Pro Software). Leveraging this novel technology, researchers can: Add western blot detection to their microplate reader within 2 minutes. Manufacturers try to design their instruments so they can be. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. Explore a wealth of applications with the future-ready SpectraMax i3x. Learn how you can perform flash-based applications, such as dual luciferase and ATP assays, with high precision and accuracy using this cartridge. *On the SpectraMax i3x reader only. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. 세포신호전달은 세포가 환경에 반응하고 다른 세포와 소통할 수 있게 합니다…. Introduction. My proficiency in techniques such as •PBMC isolation, counting and. Select PMT gain o PMT gain = energy used by detector to collect signal o Low gain for samples with high expected signalCapture flash assays with ease The SpectraMax i3x Injector Cartridge with SmartInject® Technology expands your research capabilities to include flash-based applications including dual luciferase and ATP assays. Easily upgrade or switch between detection modes with user-exchangeable modules – just open the drawer and insert the module you want. Explore a wealth of applications with the future-ready SpectraMax i3x. 5577 Web: Email: info@moldev. Figure 1. SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the dataSpectraMax i3x Multi-Mode Microplate Reader. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise: Trinean: DropSense96: AMBIENT TEMPERATURE STORAGE: Liconic: LPX 220 Hotel: LPX 440 Hotel: Thermo Fisher Scientific: Cytomat 10 Hotel: Cytomat 2 Hotel: Cytomat 24 Hotel: Cytomat 8 Linear Hotel: Cytomat 9 Hotel (MPH) BAR CODE. Itemploysa luciferaselabel(where“label”generallymeansfusedbytheuseofexpressionvectors)forthe oneproteinandaGreenFluorescentProtein(GFP)variantlabelfortheother. SpectraMax i3x Multi-Mode Detection Platform. 304 signal level saturation. 304 signal level saturation. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for. This process should take about 3 - 5 minutes. Sensitive TRF detection of two simple, mix-and-measure assay workflows. SpectraMax® Microplate Readers and SoftMax® Pro Software. ü; ü. Contact Us Phone: +1. Unpacking and setting up the instrument includes the following procedures: • Unpacking the instrument and saving the original packaging. Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. The dual auto injector module. i3x. 2. The possibility of user upgrading the system with an application cartridge and an imaging cytometer makes this device the most flexible microplate. Spectramax i3 manualSpectramax i3 manual Microplate spectramax i3x molecular biocompareSpectramax i3. The plate was then read on the SpectraMax iD5, SpectraMax i3x, and SpectraMax M5e readers using the settings in Table 1. The ScanLater Western Blot Detection Cartridge is a user-installable option for the SpectraMax i3, i3x and Paradigm Multi-Mode Readers. SpectraMax iD3 reader with touchscreen and inserted USB flash drive. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Price from $9. 10. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with. 00800 665 32860. i3x Multi-Mode Microplate Reader. Materials and methodsSpectraMax i3x Multi-Mode Microplate Reader. With the QuickSync client installed on your computer, your SpectraMax iD3 reader can push data to any computer within the same network. The spectra analysis and K d were determined using Sigmaplot 14. SpectraMax i3x Multi-Mode Detection Platform User Guide. It allows you to explore cellular pathways and protein activation and expression in one system. infoboxeu@moldev. Description 1. pathways and protein activation and expression in one system. Specifically, we present neurite. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. . We would like to show you a description here but the site won’t allow us. Measured using the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. . ZERO BIAS - scores, article reviews, protocol conditions and more. ImageXpress Confocal HT. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. Description 1. . By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. We would like to show you a description here but the site won’t allow us. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 72 5039228D. . Data Analysis. Unlimited breadth of. SpectraMax i3, i3x, and iD5 readers. .